Description
Intended Uses: This ELISA kit applies to the invitro quantitative determination of CCK-8concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with CCK-8. During the reaction,CCK-8 in the sample or standard competes with a fixed amount ofCCK-8 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CCK-8. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration ofCCK-8 in the samples is then determined by comparing the OD of the samples to the standard curve